ATG5 antibody

Catalog No. ABIN7201349

This Mouse Monoclonal antibody specifically detects ATG5 in WB, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for ATG5 antibody (ABIN7201349)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 3C7

Product Details anti-ATG5 Antibody

Purpose: Mouse Anti-ATG5 Mouse Monoclonal Antibody (3C7)

Specificity: The antibody detects endogenous ATG5 protein.

Purification: Protein G

Immunogen: Recombinant Protein of ATG5

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IF 1:100-500,ELISA 1:1000-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: Autophagy protein 5, APG5-like, Apoptosis-specific proteinATG5, also named as APG5L and ASP, belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection.

Molecular Weight: 55kD

Gene ID: 9494

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy