ATP6V1B1 antibody (AA 350-430)

Catalog No. ABIN7217472

The Rabbit Polyclonal anti-ATP6V1B1 antibody is suitable to detect ATP6V1B1 in samples from Human and Mouse. It has been validated for WB, ELISA and IHC (p).

Quick Overview for ATP6V1B1 antibody (AA 350-430) (ABIN7217472)

Target: ATP6V1B1 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1 (ATP6V1B1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP6V1B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATP6V1B1 Antibody

Binding Specificity: AA 350-430

Purpose: V-ATPase B1 Polyclonal Antibody

Specificity: V-ATPase B1 Polyclonal Antibody detects endogenous levels of V-ATPase B1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human V-ATPase B1 at AA range: 350-430

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ATP6V1B1

Target: ATP6V1B1 (ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1 (ATP6V1B1))

Alternative Name: V-ATPase B1

Background: Rabbit Anti-V-ATPase B1 Polyclonal Antibody,ATP6V1B1, ATP6B1, VATB, VPP3, V-type proton ATPase subunit B, kidney isoform, V-ATPase subunit B 1, Endomembrane proton pump 58 kDa subunit, Vacuolar proton pump subunit B 1,ATP6V1B1 encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. V-type proton ATPase subunit B kidney isoform is one of two V1 domain B subunit isoforms and is found in the kidney. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness.,V-type proton ATPase subunit B kidney isoform

Molecular Weight: observerd band 60kDa

Gene ID: 525

UniProt: P15313

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis, Proton Transport