Aurora Kinase B antibody (pTyr12)

Catalog No. ABIN7225882

This Rabbit Polyclonal antibody specifically detects Aurora Kinase B in IHC, WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Aurora Kinase B antibody (pTyr12) (ABIN7225882)

Target: Aurora Kinase B (AURKB)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-Aurora Kinase B Antibody

Binding Specificity: pTyr12

Purpose: Rabbit Anti-ARK-2 (phospho Tyr12) Polyclonal Antibody

Specificity: Phospho-ARK-2 (Y12) Polyclonal Antibody detects endogenous levels of ARK-2 protein only when phosphorylated at Y12.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human ARK-2 Phospho-Tyr12

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: ARK-2

Background: AURKB, AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5, Aurora kinase B, Aurora 1, Aurora- and IPL1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, ARK-2, Aurora-related kinase 2, STK-1, Serine/threonine-protein kinase 12AURKB (aurora kinase B) encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of AURKB is located on chromosome 8. Alternatively spliced transcript variants have been found for AURKB.

Molecular Weight: 39kD

Gene ID: 9212

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades