BCL6 antibody (pSer333)

Catalog No. ABIN7224024

The Rabbit Polyclonal anti-BCL6 antibody is suitable to detect BCL6 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for BCL6 antibody (pSer333) (ABIN7224024)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BCL6 Antibody

Binding Specificity: pSer333

Purpose: Rabbit Anti-Bcl-6 (phospho Ser333) Polyclonal Antibody

Specificity: Phospho-Bcl-6 (S333) Polyclonal Antibody detects endogenous levels of Bcl-6 protein only when phosphorylated at S333.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Bcl-6 Phospho-Ser333

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: Bcl-6

Background: BCL6, BCL5, LAZ3, ZBTB27, ZNF51, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, BCL-5, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51The protein encoded by BCL6 (B-cell CLL/lymphoma 6) is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. BCL6 is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for BCL6.

Molecular Weight: 79kD

Gene ID: 604

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus