BCL6 antibody

Catalog No. ABIN7224028

This Rabbit Polyclonal antibody specifically detects BCL6 in IHC, WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BCL6 antibody (ABIN7224028)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-BCL6 Antibody

Purpose: Rabbit Anti-Bcl-6 Polyclonal Antibody

Specificity: Bcl-6 Polyclonal Antibody detects endogenous levels of Bcl-6 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Bcl-6 around the non-phosphorylation site of S333

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: Bcl-6

Background: BCL6, BCL5, LAZ3, ZBTB27, ZNF51, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, BCL-5, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51The protein encoded by BCL6 (B-cell CLL/lymphoma 6) is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. BCL6 is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for BCL6.

Molecular Weight: 90kD

Gene ID: 604

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus