BRCA1 antibody (pSer1524)

Catalog No. ABIN7224456

This anti-BRCA1 antibody is a Rabbit Polyclonal antibody detecting BRCA1 in ELISA and IHC (p). Suitable for Human.

Quick Overview for BRCA1 antibody (pSer1524) (ABIN7224456)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BRCA1 Antibody

Binding Specificity: pSer1524

Purpose: BRCA1 (phospho Ser1524) Polyclonal Antibody

Specificity: Phospho-BRCA1 (S1524) Polyclonal Antibody detects endogenous levels of BRCA1 protein only when phosphorylated at S1524.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human BRCA1 Phospho-Ser1524

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Alternative Name: BRCA1

Background: Rabbit Anti-BRCA1 (phospho Ser1524) Polyclonal Antibody,BRCA1, RNF53, Breast cancer type 1 susceptibility protein, RING finger protein 53,BRCA1 (BRCA1, DNA repair associated) encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in BRCA1 are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for BRCA1, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified.,Breast cancer type 1 susceptibility protein

Gene ID: 672

UniProt: P38398

Pathways: Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus