BRCA2 antibody (AA 1-80)

Catalog No. ABIN7224500

The Rabbit Polyclonal anti-BRCA2 antibody is suitable to detect BRCA2 in samples from Human and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for BRCA2 antibody (AA 1-80) (ABIN7224500)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-BRCA2 Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-BRCA2 Polyclonal Antibody

Specificity: BRCA2 Polyclonal Antibody detects endogenous levels of BRCA2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human BRCA2 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2

Background: BRCA2, FACD, FANCD1, Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 proteinInherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.

Gene ID: 675

UniProt: P51587

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location