BTK antibody (pTyr551)

Catalog No. ABIN7213156

This Rabbit Polyclonal antibody specifically detects BTK in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BTK antibody (pTyr551) (ABIN7213156)

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BTK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BTK Antibody

Binding Specificity: pTyr551

Purpose: Rabbit Anti-Btk (phospho Tyr551) Polyclonal Antibody

Specificity: Phospho-Btk (Y551) Polyclonal Antibody detects endogenous levels of Btk protein only when phosphorylated at Y551.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Btk Phospho-Tyr551

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BTK

Target: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Alternative Name: Btk

Background: BTK, AGMX1, ATK, BPK, Tyrosine-protein kinase BTK, Agammaglobulinaemia tyrosine kinase, ATK, B-cell progenitor kinase, BPK, Bruton tyrosine kinaseBruton tyrosine kinase encoded by BTK plays a crucial role in B-cell development. Mutations in BTK cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 80kD

Gene ID: 695

UniProt: Q06187

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling