Calnexin antibody (pSer583)

Catalog No. ABIN7212898

This Rabbit Polyclonal antibody specifically detects Calnexin in WB, IF, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Calnexin antibody (pSer583) (ABIN7212898)

Target: Calnexin (CANX)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Calnexin Antibody

Binding Specificity: pSer583

Purpose: Calnexin (phospho Ser583) Polyclonal Antibody

Specificity: Phospho-Calnexin (S583) Polyclonal Antibody detects endogenous levels of Calnexin protein only when phosphorylated at S583.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Calnexin Phospho-Ser583

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: Calnexin

Background: Rabbit Anti-Calnexin (phospho Ser583) Polyclonal Antibody,CANX, Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90,CANX encodes a member of the calnexin family of molecular chaperones. Calnexin is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described,Calnexin

Molecular Weight: observerd band 90kDa

Gene ID: 821

UniProt: P27824

Pathways: MAPK Signaling, Thyroid Hormone Synthesis