CA12 antibody (AA 200-280)

Catalog No. ABIN7225006

This Rabbit Polyclonal antibody specifically detects CA12 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CA12 antibody (AA 200-280) (ABIN7225006)

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CA12 Antibody

Binding Specificity: AA 200-280

Purpose: Rabbit Anti-CA XII Polyclonal Antibody

Specificity: CA XII Polyclonal Antibody detects endogenous levels of CA XII protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CA XII at AA range: 200-280

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-1:2000,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CA12

Target: CA12 (Carbonic Anhydrase 12 (CA12))

Alternative Name: CA XII

Background: CA12, Carbonic anhydrase 12, Carbonate dehydratase XII, Carbonic anhydrase XII, CA-XII, Tumor antigen HOM-RCC-3.1.3Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. The gene product CA12 (carbonic anhydrase 12) is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10 % of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for CA12.

Molecular Weight: 40kD

Gene ID: 771

UniProt: O43570