CA5B antibody (AA 210-290)

Catalog No. ABIN7219286

The Rabbit Polyclonal anti-CA5B antibody (ABIN7219286) specifically detects CA5B in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CA5B antibody (AA 210-290) (ABIN7219286)

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA5B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CA5B Antibody

Binding Specificity: AA 210-290

Purpose: Rabbit Anti-CA VB Polyclonal Antibody

Specificity: CA VB Polyclonal Antibody detects endogenous levels of CA VB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human CA VB at AA range: 210-290

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CA5B

Target: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Alternative Name: CA VB

Background: CA5B, Carbonic anhydrase 5B, mitochondrial, Carbonate dehydratase VB, Carbonic anhydrase VB, CA-VBCarbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA VA. It has a wider tissue distribution than CA VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles.

Molecular Weight: 38kD

Gene ID: 11238

UniProt: Q9Y2D0