Catalase antibody (pTyr386)

Catalog No. ABIN7225452

This Rabbit Polyclonal antibody specifically detects Catalase in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Catalase antibody (pTyr386) (ABIN7225452)

Target: Catalase (CAT)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Catalase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Catalase Antibody

Binding Specificity: pTyr386

Purpose: Rabbit Anti-Catalase (phospho Tyr386) Polyclonal Antibody

Specificity: Phospho-Catalase (Y386) Polyclonal Antibody detects endogenous levels of Catalase protein only when phosphorylated at Y386.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Catalase Phospho-Tyr386

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: Catalase

Background: CAT, CatalaseCAT (catalase) encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in CAT have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by CAT.

Molecular Weight: 59kD

Gene ID: 847

UniProt: P04040

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism