CD177 antibody (Internal Region)

Catalog No. ABIN7218102

This Rabbit Polyclonal antibody specifically detects CD177 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for CD177 antibody (Internal Region) (ABIN7218102)

Target: CD177 (CD177 Molecule (CD177))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD177 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CD177 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-CD177 Polyclonal Antibody

Specificity: CD177 Polyclonal Antibody detects endogenous levels of CD177 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CD177

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD177

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177

Background: CD177, NB1, PRV1, CD177 antigen, Human neutrophil alloantigen 2a, HNA-2a, NB1 glycoprotein, NB1 GP, Polycythemia rubra vera protein 1, PRV-1, CD177CD177 encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified.

Molecular Weight: 46kD

Gene ID: 57126

UniProt: Q8N6Q3