CD247 antibody (pTyr142)

Catalog No. ABIN7225868

The Rabbit Polyclonal anti-CD247 antibody is suitable to detect CD247 in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CD247 antibody (pTyr142) (ABIN7225868)

Target: CD247 (CD247 Molecule (CD247))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD247 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD247 Antibody

Binding Specificity: pTyr142

Purpose: Rabbit Anti-CD3 ζ (phospho Tyr142) Polyclonal Antibody

Specificity: Phospho-CD3 ζ (Y142) Polyclonal Antibody detects endogenous levels of CD3 ζ protein only when phosphorylated at Y142.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CD3 zeta Phospho-Tyr142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD3 zeta

Background: CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD antigen CD247The protein encoded by CD247 (CD247 Molecule) is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247.

Molecular Weight: 20kD

Gene ID: 919

UniProt: P20963

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway