CD247 antibody

Catalog No. ABIN7225870

The Rabbit Polyclonal anti-CD247 antibody (ABIN7225870) specifically detects CD247 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for CD247 antibody (ABIN7225870)

Target: CD247 (CD247 Molecule (CD247))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD247 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD247 Antibody

Purpose: Rabbit Anti-CD3 ζ Polyclonal Antibody

Specificity: CD3 ζ Polyclonal Antibody detects endogenous levels of CD3 ζ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CD3 zeta around the non-phosphorylation site of Y142

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD247

Target: CD247 (CD247 Molecule (CD247))

Alternative Name: CD3 zeta

Background: CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD antigen CD247The protein encoded by CD247 (CD247 Molecule) is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for CD247.

Molecular Weight: 20kD

Gene ID: 919

UniProt: P20963

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway