CD28 antibody (pTyr218)

Catalog No. ABIN7225964

This Rabbit Polyclonal antibody specifically detects CD28 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD28 antibody (pTyr218) (ABIN7225964)

Target: CD28

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD28 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD28 Antibody

Binding Specificity: pTyr218

Purpose: Rabbit Anti-CD28 (phospho Tyr218) Polyclonal Antibody

Specificity: Phospho-CD28 (Y218) Polyclonal Antibody detects endogenous levels of CD28 protein only when phosphorylated at Y218.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CD28 Phospho-Tyr218

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD28

Target: CD28

Alternative Name: CD28

Background: CD28, T-cell-specific surface glycoprotein CD28, TP44, CD antigen CD28The protein encoded by CD28 (CD28 Molecule) is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for CD28.

Molecular Weight: 45kD

Gene ID: 940

UniProt: P10747

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response