CD8B antibody

Catalog No. ABIN7227770

The Rabbit Polyclonal anti-CD8B antibody is suitable to detect CD8B in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for CD8B antibody (ABIN7227770)

Target: CD8B (CD8b Molecule (CD8B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD8B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD8B Antibody

Purpose: Rabbit Anti-CD8B Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CD8B protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human CD8B protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD8B

Target: CD8B (CD8b Molecule (CD8B))

Alternative Name: CD8B

Background: T-cell surface glycoprotein CD8 beta chain, CD8bThe CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

Molecular Weight: 23kD

Gene ID: 926

UniProt: P10966

Pathways: TCR Signaling