CDK12 antibody (AA 50-130)

Catalog No. ABIN7227782

The Rabbit Polyclonal anti-CDK12 antibody is suitable to detect CDK12 in samples from Human and Rat. It has been validated for ELISA and WB.

Quick Overview for CDK12 antibody (AA 50-130) (ABIN7227782)

Target: CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-CDK12 Antibody

Binding Specificity: AA 50-130

Purpose: Rabbit Anti-CDK12 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CDK12 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human CDK12 protein at AA range: 50-130

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDK12

Target: CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))

Alternative Name: CDK12

Background: Cyclin-dependent kinase 12, Cdc2-related kinase, arginine/serine-rich, CrkRS, Cell division cycle 2-related protein kinase 7, CDC2-related protein kinase 7, Cell division protein kinase 12, hCDK12CDK12 (Cyclin Dependent Kinase 12) is a Protein Coding gene. Among its related pathways are DNA Damage and Formation of HIV elongation complex in the absence of HIV Tat. Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates Ser-5 in CTD repeats that are already phosphorylated at Ser-7, but can also phosphorylate Ser-2. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.

Molecular Weight: 163kD

Gene ID: 51755

UniProt: Q9NYV4