CDC42EP5 antibody (AA 1-80)

Catalog No. ABIN7214112

The Rabbit Polyclonal anti-CDC42EP5 antibody is suitable to detect CDC42EP5 in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CDC42EP5 antibody (AA 1-80) (ABIN7214112)

Target: CDC42EP5 (CDC42 Effector Protein (Rho GTPase Binding) 5 (CDC42EP5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42EP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDC42EP5 Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-Cdc42EP5 Polyclonal Antibody

Specificity: Cdc42EP5 Polyclonal Antibody detects endogenous levels of Cdc42EP5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Cdc42EP5 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDC42EP5

Target: CDC42EP5 (CDC42 Effector Protein (Rho GTPase Binding) 5 (CDC42EP5))

Alternative Name: Cdc42EP5

Background: CDC42EP5, BORG3, CEP5, Cdc42 effector protein 5, Binder of Rho GTPases 3Cell division control protein 42 (CDC42), a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg (binder of Rho GTPases) family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to CDC42 and regulate its function negatively. The encoded protein may inhibit c-Jun N-terminal kinase (JNK) independently of CDC42 binding. The protein may also play a role in septin organization and inducing pseudopodia formation in fibroblasts.

Molecular Weight: 22kD

Gene ID: 148170

UniProt: Q6NZY7

Pathways: Regulation of Actin Filament Polymerization