CDK1/2/3 (pThr14) antibody

Catalog No. ABIN7212914

The Rabbit Polyclonal anti-CDK1/2/3 antibody (ABIN7212914) specifically detects CDK1/2/3 in WB, IF and ELISA. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for CDK1/2/3 (pThr14) antibody (ABIN7212914)

Target: CDK1/2/3

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details

Binding Specificity: pThr14

Purpose: Rabbit Anti-Cdk1/2/3 (phospho Thr14) Polyclonal Antibody

Specificity: Phospho-Cdk1/2/3 (T14) Polyclonal Antibody detects endogenous levels of Cdk1/2/3 protein only when phosphorylated at T14.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cdk1/2/3 Phospho-Thr14

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: CDK1/2/3

Background: CDK1, CDC2, CDC28A, CDKN1, P34CDC2, Cyclin-dependent kinase 1, CDK1, Cell division control protein 2 homolog, Cell division protein kinase 1, p34 protein kinase, CDK2, CDKN2, Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 prCDK2 encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. Cyclin dependent kinase 2 is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants.

Molecular Weight: 34kD

Gene ID: 1017

UniProt: P06493, P24941, Q00526