Coagulation Factor IX antibody (AA 412-461)

Catalog No. ABIN7226924

The Rabbit Polyclonal anti-Coagulation Factor IX antibody (ABIN7226924) specifically detects Coagulation Factor IX in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Coagulation Factor IX antibody (AA 412-461) (ABIN7226924)

Target: Coagulation Factor IX (F9)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Coagulation Factor IX antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Coagulation Factor IX Antibody

Binding Specificity: AA 412-461

Purpose: Rabbit Anti-Factor IX Polyclonal Antibody

Specificity: Factor IX Polyclonal Antibody detects endogenous levels of Factor IX.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from Factor IX at AA range: 412-461

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Coagulation Factor IX

Target: Coagulation Factor IX (F9)

Alternative Name: Factor IX

Background: Coagulation factor IX, Christmas factor, Plasma thromboplastin component, PTCF9(coagulation factor IX) encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of F9, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing.

Molecular Weight: 52kD

Gene ID: 2158

UniProt: P00740