Girdin antibody

Catalog No. ABIN7223462

The Rabbit Polyclonal anti-Girdin antibody is suitable to detect Girdin in samples from Human and Mouse. It has been validated for ELISA, IHC and IF.

Quick Overview for Girdin antibody (ABIN7223462)

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Girdin antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Girdin Antibody

Purpose: Rabbit Anti-Girdin Polyclonal Antibody

Specificity: Girdin Polyclonal Antibody detects endogenous levels of Girdin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Girdin around the non-phosphorylation site of S1417

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Girdin

Target: Girdin (CCDC88A) (Coiled-Coil Domain Containing 88A (CCDC88A))

Alternative Name: Girdin

Background: CCDC88A, APE, GRDN, KIAA1212, Girdin, Akt phosphorylation enhancer, APE, Coiled-coil domain-containing protein 88A, G alpha-interacting vesicle-associated protein, GIV, Girders of actin filament, Hook-related protein 1, HkRP1CCDC88A (coiled-coil domain containing 88A) encodes a member of the Girdin family of coiled-coil domain containing proteins. The encoded protein is an actin-binding protein that is activated by the serine/threonine kinase Akt and plays a role in cytoskeleton remodeling and cell migration. The encoded protein also enhances Akt signaling by mediating phosphoinositide 3-kinase (PI3K)-dependent activation of Akt by growth factor receptor tyrosine kinases and G protein-coupled receptors. Increased expression of CCDC88A and phosphorylation of the encoded protein may play a role in cancer metastasis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Gene ID: 55704

UniProt: Q3V6T2