CD35 antibody

Catalog No. ABIN7226820

The Rabbit Polyclonal anti-CD35 antibody (ABIN7226820) specifically detects CD35 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CD35 antibody (ABIN7226820)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD35 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD35 Antibody

Purpose: Rabbit Anti-CD35 Polyclonal Antibody

Specificity: CD35 Polyclonal Antibody detects endogenous levels of CD35.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from CD35 at AA range: Internal

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:10000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Alternative Name: CD35

Background: Complement component (3b/4b) receptor 1/2 (Knops blood group)CR1 (complement C3b/C4b receptor 1 (Knops blood group)) is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. CR1 encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in CR1 have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants, encoding different isoforms, have been characterized. Additional allele specific isoforms, including a secreted form, have been described but have not been fully characterized.

Molecular Weight: 220kD

Gene ID: 1378

UniProt: P17927, Q2VPA4

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity