Cortactin antibody (pTyr466)

Catalog No. ABIN7213290

This Rabbit Polyclonal antibody specifically detects Cortactin in WB, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for Cortactin antibody (pTyr466) (ABIN7213290)

Target: Cortactin (CTTN)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cortactin Antibody

Binding Specificity: pTyr466

Purpose: Cortactin (phospho Tyr466) Polyclonal Antibody

Specificity: Phospho-Cortactin (Y466) Polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Y466.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cortactin Phospho-Tyr466

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: Cortactin

Background: Rabbit Anti-Cortactin (phospho Tyr466) Polyclonal Antibody,CTTN, EMS1, Src substrate cortactin, Amplaxin, Oncogene EMS1,Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phennotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing.,Src substrate cortactin

Molecular Weight: observerd band 80kDa

Gene ID: 13043

UniProt: Q14247

Pathways: MAPK Signaling