Cullin 5 antibody (AA 560-640)

Catalog No. ABIN7228100

This Rabbit Polyclonal antibody specifically detects Cullin 5 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cullin 5 antibody (AA 560-640) (ABIN7228100)

Target: Cullin 5 (CUL5)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cullin 5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cullin 5 Antibody

Binding Specificity: AA 560-640

Purpose: Rabbit Anti-CUL5 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CUL5 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human CUL5 protein at AA range: 560-640

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cullin 5

Target: Cullin 5 (CUL5)

Alternative Name: CUL5

Background: Cullin-5, CUL-5, Vasopressin-activated calcium-mobilizing receptor 1, VACM-1Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS (SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor.

Molecular Weight: 85kD

Gene ID: 8065

UniProt: Q93034

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development