Cyclin A antibody (AA 190-270)

Catalog No. ABIN7214390

This Rabbit Polyclonal antibody specifically detects Cyclin A in WB, IF, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for Cyclin A antibody (AA 190-270) (ABIN7214390)

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Cyclin A Antibody

Binding Specificity: AA 190-270

Purpose: Cyclin A Polyclonal Antibody

Specificity: Cyclin A Polyclonal Antibody detects endogenous levels of Cyclin A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Cyclin A at AA range: 190-270

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Cyclin A

Target: Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

Alternative Name: Cyclin A

Background: Rabbit Anti-Cyclin A Polyclonal Antibody,CCNA1, Cyclin-A1, CCNA2, CCN1, CCNA, Cyclin-A2, Cyclin-A,Cyclin A1 encoded by CCNA1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin encoded by this gene was shown to be expressed in testis and brain, as well as in several leukemic cell lines, and is thought to primarily function in the control of the germline meiotic cell cycle. This cyclin binds both CDK2 and CDC2 kinases, which give two distinct kinase activities, one appearing in S phase, the other in G2, and thus regulate separate functions in cell cycle. This cyclin was found to bind to important cell cycle regulators, such as Rb family proteins, transcription factor E2F-1, and the p21 family proteins. Multiple transcript variants encoding different isoforms have been found for CCNA1.,Cyclin-A1

Molecular Weight: observerd band 53kDa

Gene ID: 8900, 890

Pathways: PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA