CDK9 antibody (pThr186)

Catalog No. ABIN7218928

This anti-CDK9 antibody is a Rabbit Polyclonal antibody detecting CDK9 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for CDK9 antibody (pThr186) (ABIN7218928)

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDK9 Antibody

Binding Specificity: pThr186

Purpose: Cdk9 (phospho Thr186) Polyclonal Antibody

Specificity: Phospho-Cdk9 (T186) Polyclonal Antibody detects endogenous levels of Cdk9 protein only when phosphorylated at T186.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cdk9 Phospho-Thr186

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CDK9

Target: CDK9 (Cyclin-Dependent Kinase 9 (CDK9))

Alternative Name: Cdk9

Background: Rabbit Anti-Cdk9 (phospho Thr186) Polyclonal Antibody,CDK9, CDC2L4, TAK, Cyclin-dependent kinase 9, C-2K, Cell division cycle 2-like protein kinase 4, Cell division protein kinase 9, Serine/threonine-protein kinase PITALRE, Tat-associated kinase complex catalytic subunit,The cyclin dependent kinase 9 encoded by CDK9 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.,Cyclin-dependent kinase 9

Molecular Weight: observerd band 42kDa

Gene ID: 1025

UniProt: P50750

Pathways: Cell Division Cycle