CYB5R3 antibody (AA 110-190)

Catalog No. ABIN7214388

The Rabbit Polyclonal anti-CYB5R3 antibody is suitable to detect CYB5R3 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CYB5R3 antibody (AA 110-190) (ABIN7214388)

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYB5R3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CYB5R3 Antibody

Binding Specificity: AA 110-190

Purpose: Rabbit Anti-CYB5R3 Polyclonal Antibody

Specificity: CYB5R3 Polyclonal Antibody detects endogenous levels of CYB5R3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CYB5R3 at AA range: 110-190

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CYB5R3

Target: CYB5R3 (Cytochrome B5 Reductase 3 (CYB5R3))

Alternative Name: CYB5R3

Background: CYB5R3, DIA1, NADH-cytochrome b5 reductase 3, B5R, Cytochrome b5 reductase, Diaphorase-1CYB5R3 encodes cytochrome b5 reductase, which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains, while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in CYB5R3 cause methemoglobinemias.

Molecular Weight: 34kD

Gene ID: 1727

UniProt: P00387

Pathways: SARS-CoV-2 Protein Interactome