DAP Kinase 1 antibody

Catalog No. ABIN7219968

This Rabbit Polyclonal antibody specifically detects DAP Kinase 1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for DAP Kinase 1 antibody (ABIN7219968)

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAP Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DAP Kinase 1 Antibody

Purpose: Rabbit Anti-DAPK1 Polyclonal Antibody

Specificity: DAPK1 Polyclonal Antibody detects endogenous levels of DAPK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human DAPK1 around the non-phosphorylation site of S308

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DAP Kinase 1

Target: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Alternative Name: DAPK1

Background: DAPK1, DAPK, Death-associated protein kinase 1, DAP kinase 1Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants.

Molecular Weight: 160kD

Gene ID: 1612

UniProt: P53355

Pathways: MAPK Signaling, Interferon-gamma Pathway