DAXX antibody (pSer668)

Catalog No. ABIN7219974

The Rabbit Polyclonal anti-DAXX antibody is suitable to detect DAXX in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for DAXX antibody (pSer668) (ABIN7219974)

Target: DAXX (Death-Domain Associated Protein (DAXX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAXX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DAXX Antibody

Binding Specificity: pSer668

Purpose: Rabbit Anti-Daxx (phospho Ser668) Polyclonal Antibody

Specificity: Phospho-Daxx (S668) Polyclonal Antibody detects endogenous levels of Daxx protein only when phosphorylated at S668.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Daxx Phospho-Ser668

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DAXX

Target: DAXX (Death-Domain Associated Protein (DAXX))

Alternative Name: Daxx

Background: DAXX, BING2, DAP6, Death domain-associated protein 6, Daxx, hDaxx, ETS1-associated protein 1, EAP1, Fas death domain-associated proteinDAXX encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, Death domain-associated protein 6 functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.

Molecular Weight: 85-115kD

Gene ID: 1616

UniProt: Q9UER7

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway