DEFa3 antibody (Internal Region)

Catalog No. ABIN7220016

The Rabbit Polyclonal anti-DEFa3 antibody is suitable to detect DEFa3 in samples from Human and Mouse. It has been validated for IHC, ELISA and IF.

Quick Overview for DEFa3 antibody (Internal Region) (ABIN7220016)

Target: DEFa3 (Defensin, alpha 3, Neutrophil-Specific (DEFa3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DEFa3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-DEFa3 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-Defensin α3 Polyclonal Antibody

Specificity: Defensin α3 Polyclonal Antibody detects endogenous levels of Defensin α3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Defensin alpha3

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DEFa3

Target: DEFa3 (Defensin, alpha 3, Neutrophil-Specific (DEFa3))

Alternative Name: Defensin alpha3

Background: DEFA3, DEF3, Neutrophil defensin 3, Defensin, alpha 3, HNP-3, HP-3, HP3Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Neutrophil defensin 3 encoded by DEFA3, defensin, alpha 3, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. DEFA3 differs from defensin, alpha 1 by only one amino acid. This gene and the gene encoding defensin, alpha 1 are both subject to copy number variation.

Gene ID: 1668

UniProt: P59666

Pathways: Cellular Response to Molecule of Bacterial Origin