TdT antibody (AA 350-430)

Catalog No. ABIN7220066

This Rabbit Polyclonal antibody specifically detects TdT in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for TdT antibody (AA 350-430) (ABIN7220066)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TdT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TdT Antibody

Binding Specificity: AA 350-430

Purpose: Rabbit Anti-TdT Polyclonal Antibody

Specificity: TdT Polyclonal Antibody detects endogenous levels of TdT protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TdT at AA range: 350-430

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-1:2000,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: TdT

Background: DNTT, TDT, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferaseDNTT is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

Molecular Weight: 60kD

Gene ID: 1791

UniProt: P04053

Pathways: DNA Damage Repair