DHX58 antibody (AA 451-500)

Catalog No. ABIN7228212

The Rabbit Polyclonal anti-DHX58 antibody is suitable to detect DHX58 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for DHX58 antibody (AA 451-500) (ABIN7228212)

Target: DHX58 (DEXH (Asp-Glu-X-His) Box Polypeptide 58 (DHX58))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHX58 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DHX58 Antibody

Binding Specificity: AA 451-500

Purpose: Rabbit Anti-DHX58 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of DHX58 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human DHX58 protein at AA range: 451-500

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DHX58

Target: DHX58 (DEXH (Asp-Glu-X-His) Box Polypeptide 58 (DHX58))

Alternative Name: DHX58

Background: Probable ATP-dependent RNA helicase DHX58, Probable ATP-dependent helicase LGP2, Protein D11Lgp2 homolog, RIG-I-like receptor 3, RLR-3, RIG-I-like receptor LGP2, RLRDHX58 (DExH-Box Helicase 58) is a Protein Coding gene. Among its related pathways are RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways and Innate Immune System. GO annotations related to this gene include nucleic acid binding and helicase activity. An important paralog of this gene is IFIH1. Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5-triphosphorylated RNA, although it can recognize RNA lacking a 5-triphosphate.

Molecular Weight: 74kD

Gene ID: 79132

UniProt: Q96C10