DCLRE1C antibody (pSer516)

Catalog No. ABIN7224264

The Rabbit Polyclonal anti-DCLRE1C antibody is suitable to detect DCLRE1C in samples from Human and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for DCLRE1C antibody (pSer516) (ABIN7224264)

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DCLRE1C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-DCLRE1C Antibody

Binding Specificity: pSer516

Purpose: Rabbit Anti-Artemis (phospho Ser516) Polyclonal Antibody

Specificity: Phospho-Artemis (S516) Polyclonal Antibody detects endogenous levels of Artemis protein only when phosphorylated at S516.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Artemis Phospho-Ser516

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for DCLRE1C

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Alternative Name: Artemis

Background: DCLRE1C, ARTEMIS, ASCID, SCIDA, SNM1C, Protein artemis, DNA cross-link repair 1C protein, Protein A-SCID, SNM1 homolog C, hSNM1C, SNM1-like proteinDCLRE1C encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The encoded protein has single-strand-specific 5'-3' exonuclease activity, it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins. The protein also functions in the regulation of the cell cycle in response to DNA damage. Mutations in DCLRE1C can cause Athabascan-type severe combined immunodeficiency (SCIDA) and Omenn syndrome. Alternative splicing results in multiple transcript variants.

Molecular Weight: 78kD

Gene ID: 64421

UniProt: Q96SD1

Pathways: DNA Damage Repair