RAD51C antibody (AA 130-210)

Catalog No. ABIN7216750

The Rabbit Polyclonal anti-RAD51C antibody (ABIN7216750) specifically detects RAD51C in WB, ELISA, IHC and IF. The antibody is reactive with Human and Monkey samples.

Quick Overview for RAD51C antibody (AA 130-210) (ABIN7216750)

Target: RAD51C (DNA Repair Protein RAD51 Homolog 3 (RAD51C))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RAD51C Antibody

Binding Specificity: AA 130-210

Purpose: Rabbit Anti-Rad51C Polyclonal Antibody

Specificity: Rad51C Polyclonal Antibody detects endogenous levels of Rad51C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Rad51C at AA range: 130-210

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RAD51C

Target: RAD51C (DNA Repair Protein RAD51 Homolog 3 (RAD51C))

Alternative Name: Rad51C

Background: RAD51C, RAD51L2, DNA repair protein RAD51 homolog 3, R51H3, RAD51 homolog C, RAD51-like protein 2RAD51C is a member of the RAD51 family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51 and are known to be involved in the homologous recombination and repair of DNA. RAD51 paralog C can interact with other RAD51 paralogs and is reported to be important for Holliday junction resolution. Mutations in RAD51C are associated with Fanconi anemia-like syndrome. RAD51C is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing results in multiple transcript variants.

Molecular Weight: 50kD

Gene ID: 5889

UniProt: O43502

Pathways: DNA Damage Repair