Dual Specificity Phosphatase 3 (DUSP3) (C-Term) antibody

Catalog No. ABIN7217992

This Rabbit Polyclonal antibody specifically detects Dual Specificity Phosphatase 3 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Dual Specificity Phosphatase 3 (DUSP3) (C-Term) antibody (ABIN7217992)

Target: Dual Specificity Phosphatase 3 (DUSP3)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details

Binding Specificity: C-Term

Purpose: Rabbit Anti-VHR Polyclonal Antibody

Specificity: VHR Polyclonal Antibody detects endogenous levels of VHR protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human VHR

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Dual Specificity Phosphatase 3 (DUSP3)

Alternative Name: VHR

Background: DUSP3, VHR, Dual specificity protein phosphatase 3, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, VHRDual specificity protein phosphatase 3 encoded by DUSP3 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1.

Molecular Weight: 21kD

Gene ID: 1845

UniProt: P51452

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades