p300 antibody (pSer89)

Catalog No. ABIN7220278

This Rabbit Polyclonal antibody specifically detects p300 in ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for p300 antibody (pSer89) (ABIN7220278)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p300 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-p300 Antibody

Binding Specificity: pSer89

Purpose: p300 (phospho Ser89) Polyclonal Antibody

Specificity: Phospho-p300 (S89) Polyclonal Antibody detects endogenous levels of p300 protein only when phosphorylated at S89.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human p300 Phospho-Ser89

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: p300

Background: Rabbit Anti-p300 (phospho Ser89) Polyclonal Antibody,EP300, P300, Histone acetyltransferase p300, p300 HAT, E1A-associated protein p300,EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer.,Histone acetyltransferase p300

Gene ID: 2033

UniProt: Q09472

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size