E2F5 antibody (Internal Region)

Catalog No. ABIN7220114

This Rabbit Polyclonal antibody specifically detects E2F5 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for E2F5 antibody (Internal Region) (ABIN7220114)

Target: E2F5

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-E2F5 Antibody

Binding Specificity: Internal Region

Purpose: E2F-5 Polyclonal Antibody

Specificity: E2F-5 Polyclonal Antibody detects endogenous levels of E2F-5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human E2F-5

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for E2F5

Target: E2F5

Alternative Name: E2F-5

Background: Rabbit Anti-E2F-5 Polyclonal Antibody,E2F5, Transcription factor E2F5, E2F-5,The E2F transcription factor 5 encoded by E2F5 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms.,Transcription factor E2F5

Molecular Weight: observerd band 36kDa

Gene ID: 1875

UniProt: Q15329