EGR2 antibody (AA 340-420)

Catalog No. ABIN7214700

The Rabbit Polyclonal anti-EGR2 antibody is suitable to detect EGR2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for EGR2 antibody (AA 340-420) (ABIN7214700)

Target: EGR2 (Early Growth Response 2 (EGR2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGR2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-EGR2 Antibody

Binding Specificity: AA 340-420

Purpose: Rabbit Anti-Egr-2 Polyclonal Antibody

Specificity: Egr-2 Polyclonal Antibody detects endogenous levels of Egr-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Egr-2 at AA range: 340-420

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EGR2

Target: EGR2 (Early Growth Response 2 (EGR2))

Alternative Name: Egr-2

Background: EGR1, KROX24, ZNF225, Early growth response protein 1, EGR-1, AT225, Nerve growth factor-induced protein A, NGFI-A, Transcription factor ETR103, Transcription factor Zif268, Zinc finger protein 225, Zinc finger protein Krox-24, EGR2, KROX20Early growth response 1 encoded by EGR1 belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppressor gene.

Molecular Weight: 50kD

Gene ID: 1958, 1959

UniProt: P18146, P11161