ELK1 antibody (pThr417)

Catalog No. ABIN7220226

The Rabbit Polyclonal anti-ELK1 antibody (ABIN7220226) specifically detects ELK1 in IHC, ELISA, IP and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ELK1 antibody (pThr417) (ABIN7220226)

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-ELK1 Antibody

Binding Specificity: pThr417

Purpose: Rabbit Anti-Elk-1 (phospho Thr417) Polyclonal Antibody

Specificity: Phospho-Elk-1 (T417) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at T417.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Elk-1 Phospho-Thr417

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IP 2-5 μg:mg lysate,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ELK1

Target: ELK1 (ELK1, Member of ETS Oncogene Family (ELK1))

Alternative Name: Elk-1

Background: ELK1, ETS domain-containing protein Elk-1ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.

Gene ID: 2002

UniProt: P19419

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling