Ephrin B1 antibody (pTyr329)

Catalog No. ABIN7220128

The Rabbit Polyclonal anti-Ephrin B1 antibody is suitable to detect Ephrin B1 in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for Ephrin B1 antibody (pTyr329) (ABIN7220128)

Target: Ephrin B1 (EFNB1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ephrin B1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Ephrin B1 Antibody

Binding Specificity: pTyr329

Purpose: Rabbit Anti-Ephrin-B1/2 (phospho Tyr329) Polyclonal Antibody

Specificity: Phospho-Ephrin-B1/2 (Y329) Polyclonal Antibody detects endogenous levels of Ephrin-B1/2 protein only when phosphorylated at Y329.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Ephrin-B1/2 Phospho-Tyr329

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Ephrin B1

Target: Ephrin B1 (EFNB1)

Alternative Name: Ephrin-B1/2

Background: EFNB1, EFL3, EPLG2, LERK2, Ephrin-B1, EFL-3, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2, LERK-2, EFNB2, EPLG5, HTKL, LERK5, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-LThe ephrin B1 encoded by EFNB2 is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system.

Gene ID: 1947, 1948

UniProt: P98172, P52799

Pathways: RTK Signaling