EGFR antibody (pThr678)

Catalog No. ABIN7203630

The Rabbit Polyclonal anti-EGFR antibody is suitable to detect EGFR in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, ELISA and IF.

Quick Overview for EGFR antibody (pThr678) (ABIN7203630)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-EGFR Antibody

Binding Specificity: pThr678

Purpose: Rabbit Anti-EGFR (phospho Thr678) Polyclonal Antibody

Specificity: Phospho-EGFR (T678) Polyclonal Antibody detects endogenous levels of EGFR protein only when phosphorylated at T678.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human EGFR Phospho-Thr678

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: EGFR, ERBB, ERBB1, HER1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1Epidermal growth factor receptor encoded by EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. Epidermal growth factor receptor is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of tepidermal growth factor receptor to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in EGFR are associated with lung cancer.

Molecular Weight: 175kD

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins