EEF2 antibody

Catalog No. ABIN7214680

The Rabbit Polyclonal anti-EEF2 antibody is suitable to detect EEF2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for EEF2 antibody (ABIN7214680)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-EEF2 Antibody

Purpose: Rabbit Anti-EF-2 Polyclonal Antibody

Specificity: EF-2 Polyclonal Antibody detects endogenous levels of EF-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human EF-2 around the non-phosphorylation site of T56

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EF-2

Background: EEF2, EF2, Elongation factor 2, EF-2EEF2 encodes a member of the GTP-binding translation elongation factor family. Eukaryotic translation elongation factor 2 is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Molecular Weight: 100kD

Gene ID: 1938

UniProt: P13639

Pathways: AMPK Signaling