EIF3A antibody

Catalog No. ABIN7228376

The Rabbit Polyclonal anti-EIF3A antibody (ABIN7228376) specifically detects EIF3A in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for EIF3A antibody (ABIN7228376)

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EIF3A Antibody

Purpose: Rabbit Anti-EIF3A Polyclonal Antibody

Specificity: The antibody detects endogenous levels of EIF3A protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human EIF3A protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EIF3A

Target: EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A (EIF3A))

Alternative Name: EIF3A

Background: Eukaryotic translation initiation factor 3 subunit A, eIF3a, Eukaryotic translation initiation factor 3 subunit 10, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185EIF3A (Eukaryotic Translation Initiation Factor 3 Subunit A) is a Protein Coding gene. Diseases associated with EIF3A include Bardet-Biedl Syndrome and Cribriform Carcinoma. Among its related pathways are Viral mRNA Translation and Translational Control. Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2: GTP: methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Molecular Weight: 152kD

Gene ID: 8661

UniProt: Q14152

Pathways: Ribonucleoprotein Complex Subunit Organization