Separase antibody

Catalog No. ABIN7216974

The Rabbit Polyclonal anti-Separase antibody (ABIN7216974) specifically detects Separase in ELISA, IHC, WB and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for Separase antibody (ABIN7216974)

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Separase antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-Separase Antibody

Purpose: Rabbit Anti-Separase Polyclonal Antibody

Specificity: Separase Polyclonal Antibody detects endogenous levels of Separase protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Separase around the non-phosphorylation site of S801

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Separase

Target: Separase (ESPL1) (Extra Spindle Poles Like 1 (ESPL1))

Alternative Name: Separase

Background: ESPL1, ESP1, KIAA0165, Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, SeparaseStable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21, MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids.

Molecular Weight: 230kD

Gene ID: 9700

UniProt: Q14674