Ezrin antibody (pTyr353)

Catalog No. ABIN7224914

The Rabbit Polyclonal anti-Ezrin antibody is suitable to detect Ezrin in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Ezrin antibody (pTyr353) (ABIN7224914)

Target: Ezrin (EZR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Ezrin Antibody

Binding Specificity: pTyr353

Purpose: Rabbit Anti-Ezrin (phospho Tyr353) Polyclonal Antibody

Specificity: Phospho-Ezrin (Y353) Polyclonal Antibody detects endogenous levels of Ezrin protein only when phosphorylated at Y353.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Ezrin Phospho-Tyr353

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin

Background: EZR, VIL2, Ezrin, Cytovillin, Villin-2, p81The cytoplasmic peripheral membrane protein encoded by EZR (ezrin) functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for EZR. Alternatively spliced variants have also been described for EZR.

Molecular Weight: 69kD

Gene ID: 7430

UniProt: P15311

Pathways: Maintenance of Protein Location