FZD7 antibody (AA 20-100)

Catalog No. ABIN7214956

The Rabbit Polyclonal anti-FZD7 antibody is suitable to detect FZD7 in samples from Human and Mouse. It has been validated for WB, ELISA, FACS and IF.

Quick Overview for FZD7 antibody (AA 20-100) (ABIN7214956)

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-FZD7 Antibody

Binding Specificity: AA 20-100

Purpose: Rabbit Anti-Frizzled-7 Polyclonal Antibody

Specificity: Frizzled-7 Polyclonal Antibody detects endogenous levels of Frizzled-7 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human Frizzled-7 at AA range: 20-100

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,Flow Cyt 1:50-200,IF/ICC1:100-500,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for FZD7

Target: FZD7 (Frizzled Family Receptor 7 (FZD7))

Alternative Name: Frizzled-7

Background: FZD7, Frizzled-7, Fz-7, hFz7, FzE3Members of the frizzled gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. Frizzled-7 contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.

Molecular Weight: 64kD

Gene ID: 8324

UniProt: O75084

Pathways: WNT Signaling, Stem Cell Maintenance