GSPT1 antibody (AA 70-150)

Catalog No. ABIN7221384

The Rabbit Polyclonal anti-GSPT1 antibody is suitable to detect GSPT1 in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC, WB and IF.

Quick Overview for GSPT1 antibody (AA 70-150) (ABIN7221384)

Target: GSPT1 (G1 To S Phase Transition 1 (GSPT1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSPT1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-GSPT1 Antibody

Binding Specificity: AA 70-150

Purpose: Rabbit Anti-eRF3a Polyclonal Antibody

Specificity: ERF3a Polyclonal Antibody detects endogenous levels of eRF3a protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human eRF3a at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GSPT1

Target: GSPT1 (G1 To S Phase Transition 1 (GSPT1))

Alternative Name: eRF3a

Background: GSPT1, ERF3A, Eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, eRF3a, G1 to S phase transition protein 1 homologThe eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. GSPT1 is one subunit of eRF3[PMID: 15917414, 12923185]. It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1.

Molecular Weight: 55kD

Gene ID: 2935

UniProt: P15170