Connexin 43/GJA1 antibody (pSer265)

Catalog No. ABIN7221068

This Rabbit Polyclonal antibody specifically detects Connexin 43/GJA1 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Connexin 43/GJA1 antibody (pSer265) (ABIN7221068)

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Connexin 43/GJA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Connexin 43/GJA1 Antibody

Binding Specificity: pSer265

Purpose: Connexin 43 (phospho Ser265) Polyclonal Antibody

Specificity: Phospho-Connexin 43 (S265) Polyclonal Antibody detects endogenous levels of Connexin 43 protein only when phosphorylated at S265.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Connexin 43 Phospho-Ser265

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Connexin 43/GJA1

Target: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Alternative Name: Connexin 43

Background: Rabbit Anti-Connexin 43 (phospho Ser265) Polyclonal Antibody,GJA1, GJAL, Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein,GJA1 is a member of the connexin gene family. The gap junction protein alpha 1 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.,Gap junction alpha-1 protein

Molecular Weight: observerd band 43kDa

Gene ID: 2697

UniProt: P17302

Pathways: MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization