GJB2 antibody (Internal Region)

Catalog No. ABIN7214294

The Rabbit Polyclonal anti-GJB2 antibody (ABIN7214294) specifically detects GJB2 in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GJB2 antibody (Internal Region) (ABIN7214294)

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GJB2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GJB2 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-Connexin-26 Polyclonal Antibody

Specificity: Connexin-26 Polyclonal Antibody detects endogenous levels of Connexin-26 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Connexin-26

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for GJB2

Target: GJB2 (Gap Junction Protein, beta 2, 26kDa (GJB2))

Alternative Name: Connexin-26

Background: GJB2, Gap junction beta-2 protein, Connexin-26, Cx26GJB2 encodes a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in GJB2 are responsible for as much as 50 % of pre-lingual, recessive deafness.

Molecular Weight: 26kD

Gene ID: 2706

UniProt: P29033

Pathways: Sensory Perception of Sound, Cell-Cell Junction Organization